Abstract

Background. Geographic tongue is an oral mucosal lesion affecting the tongue. The association between geographic tongue and the mucosal microbiota in children remains unclear.

Method. To characterize the feature of lingual microbiota in pediatric geographic tongue, lingual swabs were collected from lesion sites and healthy sites of 25 patients with geographic tongue (14 males and 11 females; age 5.21 ±2.94 years) and 19 controls (10 males and 9 females; age 5.31±2.82 years). DNA was extracted and the 16S rRNA was amplificated, sequenced and analyzed.

Results. The lingual microbiota composition was significantly different between children with geographic tongue and the healthy cohort; Streptobacillus was reduced in geographic tongue, while Catonella, Bacillus and Oribacterium were overrepresented. When the lesions and the normal mucosa were compared, an increased abundance of Prevotella oris was observed.

Conclusion. Our results provided new insight into the association between oral microbiota and pediatric geographic tongue.

Keywords: geographic tongue, children, microbiota, 16S rRNA

Supplementary materials
Supplementary Tables
Supplementary Figures

Copyright and license

Copyright © 2024 The Author(s). This is an open access article distributed under the Creative Commons Attribution License (CC BY), which permits unrestricted use, distribution, and reproduction in any medium or format, provided the original work is properly cited.

How to cite

1.
You Y, He Y, Huang P. Characterization of lingual microbiota in pediatric geographic tongue. Turk J Pediatr 2024; 66: 448-456. https://doi.org/10.24953/turkjpediatr.2024.4638

References

  1. Pinto A, Haberland CM, Baker S. Pediatric soft tissue oral lesions. Dent Clin North Am 2014; 58: 437-453. https://doi.org/10.1016/j.cden.2013.12.003
  2. Ugar-Cankal D, Denizci S, Hocaoglu T. Prevalence of tongue lesions among Turkish schoolchildren. Saudi Med J 2005; 26: 1962-1967.
  3. Shulman JD. Prevalence of oral mucosal lesions in children and youths in the USA. Int J Paediatr Dent 2005; 15: 89-97. https://doi.org/10.1111/j.1365-263X.2005.00632.x
  4. Jainkittivong A, Langlais RP. Geographic tongue: clinical characteristics of 188 cases. J Contemp Dent Pract 2005; 6: 123-135.
  5. Dafar A, Çevik-Aras H, Robledo-Sierra J, Mattsson U, Jontell M. Factors associated with geographic tongue and fissured tongue. Acta Odontol Scand 2016; 74: 210-216. https://doi.org/10.3109/00016357.2015.1087046
  6. Picciani B, Santos VC, Teixeira-Souza T, et al. Investigation of the clinical features of geographic tongue: unveiling its relationship with oral psoriasis. Int J Dermatol 2017; 56: 421-427. https://doi.org/10.1111/ijd.13460
  7. Liang J, Huang P, Li H, et al. Mutations in IL36RN are associated with geographic tongue. Hum Genet 2017; 136: 241-252. https://doi.org/10.1007/s00439-016-1750-y
  8. Darling MR, Su N, Masen S, et al. Geographic tongue: assessment of peripheral nerve status, Langerhans cell, and HLA-DR expression. Oral Surg Oral Med Oral Pathol Oral Radiol 2017; 124: 371-377.e1. https://doi.org/10.1016/j.oooo.2017.05.516
  9. Wysocki GP, Daley TD. Benign migratory glossitis in patients with juvenile diabetes. Oral Surg Oral Med Oral Pathol 1987; 63: 68-70. https://doi.org/10.1016/0030-4220(87)90342-2
  10. Garsjö V, Dafar A, Jontell M, Çevik-Aras H, Bratel J. Increased levels of calprotectin in the saliva of patients with geographic tongue. Oral Dis 2020; 26: 558-565. https://doi.org/10.1111/odi.13258
  11. Dafar A, Bankvall M, Garsjö V, Jontell M, Çevik-Aras H. Salivary levels of interleukin-8 and growth factors are modulated in patients with geographic tongue. Oral Dis 2017; 23: 757-762. https://doi.org/10.1111/odi.12654
  12. Kullaa-Mikkonen A, Kotilainen R. The prevalence of oral carriers of Candida in patients with tongue abnormalities. J Dent 1983; 11: 313-317. https://doi.org/10.1016/0300-5712(83)90115-x
  13. Dudko A, Kurnatowska AJ, Kurnatowski P. Prevalence of fungi in cases of geographical and fissured tongue. Ann Parasitol 2013; 59: 113-117.
  14. Krishnan K, Chen T, Paster BJ. A practical guide to the oral microbiome and its relation to health and disease. Oral Dis 2017; 23: 276-286. https://doi.org/10.1111/odi.12509
  15. Su SC, Chang LC, Huang HD, et al. Oral microbial dysbiosis and its performance in predicting oral cancer. Carcinogenesis 2021; 42: 127-135. https://doi.org/10.1093/carcin/bgaa062
  16. Zhong X, Lu Q, Zhang Q, He Y, Wei W, Wang Y. Oral microbiota alteration associated with oral cancer and areca chewing. Oral Dis 2021; 27: 226-239. https://doi.org/10.1111/odi.13545
  17. Hernandez BY, Zhu X, Goodman MT, et al. Betel nut chewing, oral premalignant lesions, and the oral microbiome. PLoS One 2017; 12: e0172196. https://doi.org/10.1371/journal.pone.0172196
  18. Chen J, Liu K, Sun X, Shi X, Zhao G, Yang Z. Microbiome landscape of lesions and adjacent normal mucosal areas in oral lichen planus patient. Front Microbiol 2022; 13: 992065. https://doi.org/10.3389/fmicb.2022.992065
  19. Dafar A, Bankvall M, Çevik-Aras H, Jontell M, Sjöberg F. Lingual microbiota profiles of patients with geographic tongue. J Oral Microbiol 2017; 9: 1355206. https://doi.org/10.1080/20002297.2017.1355206
  20. Willis JR, Saus E, Iraola-Guzmán S, et al. Citizen-science reveals changes in the oral microbiome in Spain through age and lifestyle factors. NPJ Biofilms Microbiomes 2022; 8: 38. https://doi.org/10.1038/s41522-022-00279-y
  21. Dzidic M, Collado MC, Abrahamsson T, et al. Oral microbiome development during childhood: an ecological succession influenced by postnatal factors and associated with tooth decay. ISME J 2018; 12: 2292-2306. https://doi.org/10.1038/s41396-018-0204-z
  22. Assimakopoulos D, Patrikakos G, Fotika C, Elisaf M. Benign migratory glossitis or geographic tongue: an enigmatic oral lesion. Am J Med 2002; 113: 751-755. https://doi.org/10.1016/s0002-9343(02)01379-7
  23. Lamont RJ, Koo H, Hajishengallis G. The oral microbiota: dynamic communities and host interactions. Nat Rev Microbiol 2018; 16: 745-759. https://doi.org/10.1038/s41579-018-0089-x
  24. Diaz PI, Hoare A, Hong BY. Subgingival microbiome shifts and community dynamics in periodontal diseases. J Calif Dent Assoc 2016; 44: 421-435.
  25. Herrero ER, Fernandes S, Verspecht T, et al. Dysbiotic biofilms deregulate the periodontal inflammatory response. J Dent Res 2018; 97: 547-555. https://doi.org/10.1177/0022034517752675
  26. Nassar M, Tabib Y, Capucha T, et al. GAS6 is a key homeostatic immunological regulator of host-commensal interactions in the oral mucosa. Proc Natl Acad Sci U S A 2017; 114: E337-E346. https://doi.org/10.1073/pnas.1614926114
  27. Burcham ZM, Garneau NL, Comstock SS, et al. Patterns of oral microbiota diversity in adults and children: a crowdsourced population study. Sci Rep 2020; 10: 2133. https://doi.org/10.1038/s41598-020-59016-0
  28. Li Y, He J, He Z, et al. Phylogenetic and functional gene structure shifts of the oral microbiomes in periodontitis patients. ISME J 2014; 8: 1879-1891. https://doi.org/10.1038/ismej.2014.28
  29. Siqueira JF, Rôças IN. Catonella morbi and Granulicatella adiacens: new species in endodontic infections. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006; 102: 259-264. https://doi.org/10.1016/j.tripleo.2005.09.021
  30. Yang J, He P, Zhou M, et al. Variations in oral microbiome and its predictive functions between tumorous and healthy individuals. J Med Microbiol 2022; 71: 10.1099/jmm.0.001568. https://doi.org/10.1099/jmm.0.001568
  31. Herreros-Pomares A, Llorens C, Soriano B, et al. Oral microbiome in Proliferative Verrucous Leukoplakia exhibits loss of diversity and enrichment of pathogens. Oral Oncol 2021; 120: 105404. https://doi.org/10.1016/j.oraloncology.2021.105404
  32. Lim Y, Fukuma N, Totsika M, Kenny L, Morrison M, Punyadeera C. The performance of an oral microbiome biomarker panel in predicting oral cavity and oropharyngeal cancers. Front Cell Infect Microbiol 2018; 8: 267. https://doi.org/10.3389/fcimb.2018.00267
  33. Ye J, Cai X, Yang J, et al. Bacillus as a potential diagnostic marker for yellow tongue coating. Sci Rep 2016; 6: 32496. https://doi.org/10.1038/srep32496
  34. Monteiro MF, Altabtbaei K, Kumar PS, et al. Parents with periodontitis impact the subgingival colonization of their offspring. Sci Rep 2021; 11: 1357. https://doi.org/10.1038/s41598-020-80372-4
  35. Chen B, Wang J, Wang Y, et al. Oral microbiota dysbiosis and its association with Henoch-Schönlein Purpura in children. Int Immunopharmacol 2018; 65: 295-302. https://doi.org/10.1016/j.intimp.2018.10.017
  36. O'Connor JB, Mottlowitz MM, Wagner BD, et al. Divergence of bacterial communities in the lower airways of CF patients in early childhood. PLoS One 2021; 16: e0257838. https://doi.org/10.1371/journal.pone.0257838
  37. Fernández Vecilla D, Roche Matheus MP, Iglesias Hidalgo G, Ugalde Zárraga E, Unzaga Barañano MJ, Díaz de Tuesta Del Arco JL. Two cases of Prevotella oris causing serious pleuropulmonary infections. Rev Esp Quimioter 2023; 36: 439-441. https://doi.org/10.37201/req/001.2023
  38. Xiang Q, Tang X, Cui S, et al. Capsaicin, the spicy ingredient of chili peppers: effects on gastrointestinal tract and composition of gut microbiota at various dosages. Foods 2022; 11: 686. https://doi.org/10.3390/foods11050686