Graphical Abstract

Graphical abstract

Abstract

Background. Food-induced anaphylaxis (FIA) is a severe form of food allergy, and literature data about multi-triggered FIA is scarce. This study aimed to evaluate the differences between multi-triggered and single-triggered food anaphylaxis in children.

Methods. The study included pediatric patients (age <18 years) who were diagnosed with FIA between January 1, 2015 and February 28, 2023. Demographic data, clinical features, laboratory findings, and allergological work-up results were evaluated from the patients’ records.

Results. A total of 459 patients (64.1% male) were evaluated. The median age at onset (first anaphylactic reaction) was 12 months (IQR:6-24 months). Food anaphylaxis with multiple foods was reported in 114 of the 459 children (24.8%). Multi-triggered FIA was most commonly associated with combinations of milk and egg (n=21) and tree nut and tree nut (n=21). Atopic disease, asthma, higher total IgE level, and higher eosinophil count were more frequent in patients with multi-triggered FIA. In multivariate regression analysis, total IgE >100 IU/mL was identified as a predictive factor for multi-triggered FIA (Odds ratio (95% CI): 2.46 (1.40-4.30), p=0.001).

Conclusions. FIA with multiple trigger foods was detected in approximately a quarter of the children with FIA. Multi-triggered FIA was associated with higher rates of atopic disease, asthma, eosinophilia, and increased total IgE levels. A total IgE level higher than 100 IU/mL was a risk factor for multi-triggered FIA. This suggests that high IgE levels may be a warning sign for clinicians to be vigilant for multiple food triggers in the screening and follow-up of FIA patients.

Keywords: anaphylaxis, food induced anaphylaxis, multiple triggered, single triggered, total IgE

Introduction

Anaphylaxis is a severe, life-threating disease and foods are the main triggers of anaphylaxis in children.1-5 Food-induced anaphylaxis (FIA) can sometimes occur with more than one food, which is referred to as multi-triggered FIA. However, there is limited information about this in the literature, and the true prevalence of multi-triggered FIA remains unclear. Identifying genuine cases of anaphylaxis to multiple foods is challenging due to the severity of the reaction, fear of oral challenge testing, and/or cross-reactivity. McIntyre et al. reported that approximately a third of anaphylaxis cases occurred with multiple substances, and approximately two-thirds (66%) of the reactions in patients with multiple allergies were related to multiple foods.6

A recent study indicated that multi-food allergic individuals were epigenetically distinct from single-food allergic individuals.7 Other studies reported that patients with multiple food allergy (MFA) had more food allergy-related adverse events8, as well as more severe food-induced allergic reactions and more frequent emergency department visits.9 Patients with MFA were also reported to have an increased prevalence of atopic comorbidities and asthma morbidity10-12, while highly atopic children may be prone to having allergies to multiple foods.13 MFA can result in increased accidental ingestions and may cause fatal/near-fatal reactions.9 Several studies reported that MFA imposes a greater physical health impact, quality of life impairment, and economic burden than single food allergy (SFA).11,13,14 These impacts may be exacerbated in patients with multi-triggered FIA because of the rigid elimination requirements and later development of tolerance. Although data about multi-triggered FIA are scarce, we hypothesized that these patients may exhibit differences from those with single-triggered FIA, like in MFA.

It is known that having one atopic disease can lead to having another; thus, a SFA may predispose to additional food allergies.15 However, we are aware of no study published to date that demonstrates predictive factors for multi-triggered FIA.

In the present study, we examined the differences in the characteristics of children with FIA to single and multiple foods and assessed the value of certain factors in predicting multi-triggered FIA.

Materials and Methods

In this single-center retrospective study, children (age <18 years) who presented to our pediatric allergy and immunology clinic with FIA between 2015 and 2023 were identified by screening patient records in the hospital’s electronic database. If the records were incomplete, we contacted the patient’s family to obtain missing data.

We recorded patient data in a standardized form that included their demographic and clinical characteristics, laboratory results (eosinophil count and percentage; tryptase levels and total IgE levels at presentation), and allergological work-up results (baseline specific IgE [sIgE] levels, skin prick test (SPT) wheal diameter, and oral food challenge (OFC) results. Results closest to the anaphylaxis reaction were recorded.

FIA was defined according to the criteria established by the European Academy of Allergy and Clinical Immunology (EAACI) task force position papers on childhood anaphylaxis management.1,16 All cases were evaluated by pediatric allergology physicians based on clinical history and complementary allergy tests (skin prick test / sIgE / Tryptase levels / open food challenge) cases were deemed to involve anaphylaxis triggered by a specific food when these assessments confirmed the diagnosis. Tryptase levels were considered if the patient was admitted within six hours after the reaction or if the reaction occurred during an open food challenge. Open food challenge tests were conducted in appropriate cases, provided parental informed consent was obtained and the case was considered non severe.

Trigger foods were identified through reaction history and allergological testing, including SPT, serum sIgE levels (measured using the Immulite® 2000 System; Siemens Healthcare Diagnostics, Tarrytown, New York), and/or OFC tests. sIgE levels were regarded as positive when equal to or greater than 0.35 kU/L. SPT was performed at least four to six weeks after the anaphylactic reaction, with antihistamines discontinued one week before testing. SPT was conducted using commercial preparations (ALK, Abello, Madrid, Spain) or fresh foods (e.g., vegetables, fruit, and fish) via the prick-to-prick method following EAACI guidelines.17 SPT was performed on the back in younger children and on the inner forearm in older children. Maximum horizontal and vertical wheal diameters were measured after 15 minutes, with a reaction considered positive if the mean diameter exceeded the negative control by at least 3 mm.

After obtaining informed parental consent; open OFCs were performed in our allergy unit under the supervision of an allergist according to EAACI guidelines.17 A stepwise OFC was preferred for milk (baked, then yogurt, then milk) and eggs (baked, then boiled). In the open OFC, the culprit food was freshly prepared and administered orally in incrementally increasing doses every 15 minutes. OFCs were considered positive if objective symptoms occurred within two hours of the last challenge dose.

Trigger foods were categorized into eight groups: milk, egg, treenuts, legumes, seafood, fruits/vegetables, seeds, and other. Treenuts included hazelnuts, walnuts, cashews, almonds, and pistachios. Legumes comprised lentils, peanuts, chickpeas, beans, peas, and soy. The seeds group included sesame, poppy, sunflower, and pumpkin seeds. Fruits/vegetables included banana, kiwi, potato, peach, coconut, strawberry, tomato, apple, melon, olive, and red pepper. The “other” category encompassed honey, meats (chicken and beef), sumac, cinnamon, and wheat.

Anaphylaxis triggered by more than one food was classified as multi-triggered FIA.18 However, due to the retrospective nature of the study, cross-reactivity could not be ruled out and food-specific components could not be measured in our hospital. Nevertheless, as all included patients met EAACI anaphylaxis criteria, demonstrated clinical reactivity and had confirmed allergens based on sIgE, SPT and/or OFC, all cases of anaphylaxis involving multiple foods were included in the statistical analysis as part of the “multi-triggered FIA” group. This classification applied even when multiple tree nuts or legumes were involved.18 Patients with multiple food allergies who experienced anaphylaxis triggered by only one food were classified as having single triggered anaphylaxis.

All patients were assessed for other atopic diseases according to international guidelines by a pediatric allergy immunology specialist.19-21 Patients were also evaluated for mastocytosis based on clinical history (recurrent, venom-induced or unexplained anaphylaxis), physical examination (typical skin lesions, hepatosplenomegaly) and/or baseline tryptase levels.

Ethics

This study protocol was reviewed and approved by Ankara Bilkent City Hospital No. 2 Clinical Research Ethics Committee (date: 15.03.2023, number: E2-23-3593).

Statistics

SPSS version 22.0 (IBM Corp, Armonk, NY) was used for statistical analyses. The data were presented as median and interquartile ranges (IQR) because the data were not normally distributed. For comparisons of groups, we used the chi-squared (χ2) and Fisher’s exact tests for qualitative variables and the Mann-Whitney U and Wilcoxon rank sum tests for quantitative variables. Univariate and multivariate analysis were used to identify risk factors for multi-triggered FIA. Results with P values < 0.005 were considered statistically significant.

Results

A total of 459 patients (599 reactions) met the inclusion criteria. The median age at the time of the first anaphylactic reaction was 12 months (IQR: 6-24 months) and 64.21% (n = 294) were male. The baseline characteristics of the patients are summarized in Table I and characteristics of the reactions are delineated in Table II.

IQR, interquartile range; NA, not analyzed.
Table I. Demographic characteristics of children and comparison between anaphylaxis cases triggered by single versus multiple food allergens.
Parameters
All patients (n: 459)
Patients with single triggered food allergy
(n: 345)
Patients with multiple triggered food allergy
(n: 114)
p
Age at first anaphylaxis (months), median (IQR)
12 (6-24)
12 (6-24)
24 (12-48)
0.052
Male gender, n (%)
294 (64.1)
223 (64.6)
71 (62.2)
0.649
Atopic diseases, n (%)
365 (79.5)
267 (77.3)
98 (85.9)
0.049
Asthma
206 (44.9)
143 (41)
63 (55)
0.010
Atopic dermatitis
227 (49.5)
166 (48.1)
61 (53.5)
0.318
Allergic rhinitis
85 (18.5)
57 (16.5)
28 (24.5)
0.055
History of family atopic disease, n (%)
118 (25.7)
87 (25)
31 (27)
0.642
Multiple food allergy
301 (65.5)
187 (54.2)
114 (100)
NA
Co-anapyhylaxis with different foods, n (%)
114 (24.8)
-
114 (100)
NA
Recurrent anaphylaxis with same food, n (%)
161 (35.1)
124 (35.9)
37(33.3)
0.652
Anaphylaxis was the first symptom, n (%)
290 (63.2)
221 (64.1)
-
NA
Total IgE (IU/mL), median (IQR)
680 (49.9-560)
115 (33-404)
273 (89-872)
<0.001
Eosinophil count (×109/L), median (IQR)
400 (200-640)
360 (200-600)
480 (280-700)
0.011
GIS, gastrointestinal system; IQR: interquartile range, sIgE: specific immunoglobulin E, SPT: skin prick test.
Table II. Characteristics of reactions (n: 599).
Total reactions (n: 599)
Reactions with single triggered food (n: 345)
Reactions with multi-triggered food (n: 254)
Types of food, n (%)
Milk
178 (29.7)
137 (39.7)
41 (16.1)
Egg
114 (19)
76 (22)
38 (15)
Tree nut
156 (26)
58 (16.8)
98 (38.6)
Hazelnut
58 (9.6)
17 (4.9)
41 (16.1)
Walnut
39 (6.6)
15 (4.3)
24 (9.4)
Pistachio
34 (5.6)
18 (5.4)
16 (6.3)
Cashew
15 (2.5)
6 (1.7)
9 (3.5)
Almond
10 (1.6)
2 (0.6)
8 (3.1)
Legume
61 (10.1)
20 (5.8)
41 (16.1)
Lentil
27 (4.5)
11 (3.2)
16 (6.3)
Peanut
14 (2.3)
5 (1.5)
9 (3.5)
Chickpea
13 (2.1)
4 (1.2)
9 (3.5)
Beans
5 (0.7)
-
5 (3.5)
Peas
1 (0.1)
-
1 (0.4)
Soy
1 (0.1)
-
1 (0.4)
Fish
16 (2.6)
14 (4.1)
2 (0.8)
Fruits/vegetables
30 (5)
14 (4.1)
16 (6.8)
Banana
7 (1.1)
4 (1.2)
3 (1.2)
Kiwi
6 (1)
2 (0.6)
4 (1.6)
Potatoes
5 (0.8)
2 (0.6)
3 (1.2)
Peach
3 (0.5)
2 (0.6)
1 (0.4)
Coconut
2 (0.3)
1 (0.3)
1 (0.4)
Strawberry
2 (0.3)
1 (0.3)
1 (0.4)
Tomato
1 (0.1)
1 (0.3)
-
Apple
1 (0.1)
-
1 (0.4)
Melon
1 (0.1)
-
1 (0.4)
Red pepper
1 (0.1)
1 (0.3)
-
Olive
1 (0.1)
-
1 (0.1)
Seeds
26 (4.3)
19 (5.5)
7 (2.8)
Sesame
20 (3.3)
15 (4.3)
5 (2)
Poppy
3 (0.5)
1 (0.3)
2 (0.8)
Sun flower seed
2 (0.3)
2 (0.6)
-
Pumpkin
1 (0.1)
1 (0.3)
-
Other
18 (30)
7 (2)
11 (4.3)
Meats (5 chicken, 5 beef)
10 (1.6)
2 (0.6)
8 (3.2)
Honey
4 (0.6)
3 (0.9)
1 (0.4)
Wheat
2 (0.3)
-
2 (0.8)
Sumac
1 (0.1)
1 (0.3)
-
Cinnamon
1 (0.1)
1 (0.3)
-
Symptoms, n (%)
Skin
571 (95.3)
333 (96.5)
238 (93.7)
GIS
274 (45.7)
171 (49.6)
103 (40.6)
Respiratory
478 (79.7)
260 (75.4)
218 (85.8)
Neurological
51 (8.5)
36 (10.4)
15 (5.9)
Cardiovascular
13 (2.1)
8 (2.3)
5 (2)
Severity of anaphylaxis, n (%)
Mild
143 (23.8)
90 (26.1)
53 (20.9)
Moderate
369 (61.6)
197 (57.1)
172 (67.7)
Severe
87 (14.5)
58 (16.8)
29 (11.4)
Reaction time (minute), median (IQR)
5 (5-15)
5 (5-15)
5 (5-15)

Multi-triggered FIA

Food anaphylaxis involving more than one food was observed in 114 of the 459 children (24.8%), accounting for 254 reactions. Among these, ninety-two patients experienced anaphylactic reactions at different times with two different foods, 19 with three different foods, 2 patients with four different foods, and 1 patient with five different foods (Supplementary Table: Triggered foods in patients with multi-triggered FIA). The most common food combinations associated with multi-triggered FIA were milk/egg (n=21) and treenut/treenut (two different treenut co-anaphylaxis) (n=21; with walnut/hazelnut (n=15) being the most frequent pairing; combinations are shown in the Supplementary Table).

Children with multi-triggered FIA had higher rates of atopic disease and asthma, as well as elevated IgE and eosinophil levels compared to those with single-triggered FIA, (p= 0.049, p= 0.010, p= <0.001, p=0.010, respectively). The similarities and differences between these groups are detailed in Table I.

Multivariate regression analysis identified, a total IgE level >100 IU/mL as a risk factor for multi-triggered FIA (Odds ratio [95% CI]: 2.46 [1.40-4.30], p=0.001, Table III).

CI, confidence interval; IgE, immunglobulin E; OR, odds ratio.
Table III. Predictive factors for food-induced anaphylaxis with multiple triggering foods.
Parameters
Univariant
Multivariant
OR
CI 95%
p
OR
CI 95%
p
Gender (male)
0.90
0.58-1.40
0.649
>2 years of age
2.08
1.30-3.32
0.002
1.31
0.71-2.44
0.307
Presence of other atopic disease
1.78
0.99-3.21
0.051
1.40
0.62-3.18
0.177
Atopic dermatitis
1.24
0.81-1.89
0.318
Asthma
1.74
1.13-2.67
0.011
1.29
0.72-2.31
0.121
Allergic rhinitis
1.64
0.98-2.74
0.057
1.11
0.56-2.22
0.327
Family atopic disease history
1.10
0.68-1.78
0.67
Total IgE >100 IU/mL
2.56
1.52-4.33
0.000
2.46
1.40-4.30
0.001
Eosinophil ≥400×109/L
2.079
1.24-3.48
0.005
1.62
0.93-2.81
0.081

Severity of reactions

Among the 345 patients with single triggered FIA; and 57.1% (n=197) of reactions were moderate; 26.1% (n=90) of them were mild and 16.8 % (n= 58) were severe. In contrast, the 114 patients with multi-triggered FIA experienced 254 reactions; of which 67.7% (n=172) were moderate, 20.9 % (n:53) were mild and 11.4% (n=29) were severe.

Unusual triggers

Four patients experienced anaphylaxis after consuming honey, none of whom had pollen atopy, although one had a history of venom anaphylaxis. Five patients had anaphylaxis triggered by potatoes with all reactions occurring after consuming baked potatoes. Two patients experienced anaphylaxis triggered by red meat, both reacting to well-cooked meat despite no known exposure to ticks.

Discussion

This single-centered, real-life study, examines the differences between multi-triggered and single-triggered food anaphylaxis in children. Multi-triggered FIA was detected in 24.8% of the patients with FIA. Patients with multi-triggered FIA were more likely to have atopic disease, asthma, higher total IgE levels, and higher eosinophil counts. Additionally, a total IgE level above 100 IU/mL was associated with an increased risk of multi-triggered FIA.

Food allergens are common triggers of anaphylaxis in children.1,2,4,5 While the exact prevalence of FIA remains uncertain, non-fatal FIA reactions have been reported at an incidence of 0.5-16 per 100000 person-years.22 In a study on epinephrine administration for life-threatening allergic reactions in school settings, McIntyre et al. reported that over one-third (36%) of reactions occurred in individuals allergic to multiple substances, with approximately two-thirds (66%) of these reactions involving multiple foods.6 Although there is limited data about the prevalence of multi-triggered FIA, several studies reported MFA in 30-40% of children with food allergy.9,18,23 In the current study, 24.8% of FIA cases involved multiple trigger foods.

Individual food allergen types in FIA may vary according to culture and population.22 The most commonly reported triggers in FIA included cow’s milk, hen’s egg, peanut, tree nuts, shellfish, and fish.2 McIntyre et al. reported that among patients who reported multiple allergies, the most common allergens listed were tree nuts (54%) and peanuts (51%).6 In our study, multi-triggered FIA was most frequently associated with milk and eggs or a combination of tree nuts.

Many studies have investigated co-allergy and co-sensitization to treenuts. The most common treenut co-allergens vary by country depending on consumption patterns, with pistachio/cashew and walnut/pecan among the most frequently reported combinations.24,25 In contrast, we observed in this study that walnut and hazelnut were the most common tree nut combinations associated with multi-triggered FIA. This is not surprising, considering that walnut and hazelnut are the most consumed treenuts in our country.26 Another study from our country showed that walnut-allergic children have a higher rate of concomitant hazelnut allergy.25 Furthermore, unlike the aforementioned studies, our study focused on the most severe cases (anaphylaxis), and reaction severity may differ according to the combination of foods. Two components of hazelnut, Cor a 9 and Cor a14, are members of a protein family known to cause severe anaphylactic reactions.24

In our study, milk/egg was the most common combination of anaphylaxis-inducing foods overall. This may be attributed to the age distribution of our sample, which was predominantly composed of infants. The intake ratio of a food is a determining factor in the development of allergic reactions24, and infants typically consume milk and egg more than other types of foods. We also observed that egg in particular was the most common co-allergen food in children with multi-triggered FIA. A mouse model study illustrated that primary ovalbumin sensitization enhanced sensitization to a secondary unrelated allergen (latex).27 Our findings may support this observation. In addition, Cetinkaya et al. reported egg white as the most common co-allergen other than tree nut/peanut in patients with tree nut/peanut allergy, but only 41.4% of the patients in their study had anaphylactic reactions.25 Masthoff et al. reported that peanut allergy was common among hazelnut-sensitized patients but was not primarily due to IgE cross-reactivity. They indicated that it may be a result of having an atopic predisposition, and being exposed to tree nuts and peanuts most likely increased the risk for co-sensitization to both foods.28 They also proposed that T-cell cross-reactivity could contribute to this phenomenon.28 Further research is needed to understand why egg or tree nuts tend to cause multi-triggered FIA as observed in our study and previous investigations.

A study indicated that individuals with MFA exhibited epigenetic differences from individuals allergic to a single food, detected as variations in DNA methylation between these groups.7 Therefore, there may also be some epigenetic differences between multi-triggered FIA and single-triggered FIA. Furthermore, certain immunological differences were reported previously between single- and multi-triggered food allergies.8,27,29 It is well established that DOCK8 deficiency is likely associated with anaphylaxis with multiple foods.29 However, there is limited information about the pathophysiologic, genetic/epigenetic, and clinical differences between single-triggered and multi-triggered FIA. Understanding these differences and identifying predisposing factors for multi-triggered FIA could aid in prevention and management. Previous studies have shown that MFA is linked to greater impairment in quality of life, higher rates of concomitant allergic disease, and increased reaction severity.9,11,18 Warren et al. reported that the prevalence of physician-diagnosed atopic comorbidities increased significantly as the number of reported convincing food allergies increased.18 Wang also stated that highly atopic children may be at greater risk of developing allergies to multiple foods13, while Hill et al. reported that individuals with MFA were at increased risk of developing asthma and rhinitis compared to patients with a SFA.11 In our study, concomitant atopic disease, especially asthma was more frequent in children with multi-triggered FIA. Asthma may contribute to the development of anaphylaxis by increasing the severity of reactions. Moreover, we detected that total IgE levels and eosinophil counts were higher in children with multi-triggered FIA than in those with single-triggered FIA. Similarly, Blumchen et al. reported that total IgE levels and Th2 responses were higher in double allergen sensitized mice than single allergen sensitized mice, and that the strength of the primary sensitization is an important factor in increasing Th2 responses to the secondary allergen.27

Data about predictors of multi-triggered FIA are also scarce. In previous tree nut studies investigating multiple nut allergies, patient age was reported to be an important factor influencing the prevalence of MFA.2,24,30 It was also noted that because the number of nuts consumed increased with age, older age may be associated with multiple nut allergies.30 Çetinkaya et al. reported that a current age of 6-10 years and a family history of atopy were risk factors for multiple tree nut/peanut allergies.25 Waren et al. determined that MFA was more prevalent among non-Hispanic Black and Asian children compared to non-Hispanic White children.18 Ruran et al. suggested that social and environmental risk factors and other comorbidities may also predispose patients to develop MFA.9 As mentioned above, atopic children may have a higher risk for MFA.13 In addition, Allen et al. reported that infants with vitamin D insufficiency were more prone to developing MFA rather than SFA.29 Unlike these previous studies, we specifically investigated predictors of multi-triggered FIA, rather than MFA, and found that total IgE levels above 100 IU/mL were associated with multi-triggered FIA. This suggests that for food-allergic children with high total IgE levels, clinicians should be more vigilant for recurrent anaphylaxis with other types of foods and may follow them more closely. However, the causality of this relationship remains unclear, as elevated IgE levels may both contribute to and result from FIA, as seen in DOCK8-deficient patients. We hope that our results may guide new studies on multi-triggered FIA in terms of its prevalence, mechanisms, characteristics, and management. However, IgE levels alone are insufficient to predict multi-triggered FIA; as they are influenced by factors such as allergen exposure frequency and duration. Therefore, a large-scale cohort study is necessary to better understand these relationships.

Component testing can be useful in distinguishing between cross-reactivity and true sensitization, especially in patients with allergies to multiple foods such as nuts. However, since these tests were not performed on the patients in our study, we could not make a clear distinction between cross-reactivity and sensitization. This is the most important limitation of our study. Another limitation is that we classified patients into single- or multi-triggered groups based on the number of anaphylaxis episodes. However, confounding factors—such as protective parental behaviors (e.g., eliminating suspicious allergens from the child’s diet) or the child’s age (e.g., not yet exposed to certain foods)—may have influenced the occurrence of new anaphylaxis events, potentially limiting our findings.

Additionally, baseline serum tryptase levels were not available for all patients, which restricted our ability to assess the potential presence of systemic mastocytosis. However, systemic mastocytosis is extremely rare in children, with most pediatric cases limited to cutaneous forms that typically resolve spontaneously.31,32 None of our patients exhibited clinical features suggestive of systemic mastocytosis, such as characteristic skin lesions, hepatosplenomegaly, or unexplained recurrent anaphylaxis. In the absence of clinical signs indicating systemic involvement, we did not pursue further diagnostic evaluations, such as bone marrow biopsy, in accordance with current guidelines.32,33

The strongest aspect of this research is that it is a single-centered, real-life FIA study. Since there are a limited number of studies in the literature reporting the differences between single and multiple food anaphylaxis, we believe that our study makes a significant contribution to the literature.

Furthermore, our study highlights the considerable rate of multi-triggered FIA among anaphylaxis cases. This should encourage new studies on this subject; because further longitudinal, multi-layer, case-control and molecular-based studies are needed.

In conclusion, multi-triggered FIA was detected in approximately a quarter of children with FIA. Higher rates of atopic disease and asthma and higher levels of total IgE and eosinophilia were observed in patients with multi-triggered FIA when compared with children with single-triggered FIA. Moreover, higher total IgE was found to be a significant predictor of multi-triggered FIA.

Ethical approval

The study was approved by Ankara Bilkent City Hospital No. 2 Clinical Research Ethics Committee (date: 15.03.2023, number: E2-23-3593).

Author contribution

The authors confirm contribution to the paper as follows: Study conception and design: ŞBY, İKÇ, ZŞE, MT, EDM, EC; data collection: ŞBY, BK, TG, SAA, EDM, EC; analysis and interpretation of results: ŞBY, İKÇ, ZŞE, MT, EDM, EC; draft manuscript preparation: ŞBY, İKÇ, ZŞE, MT, EDM, EC. All authors reviewed the results and approved the final version of the manuscript.

Source of funding

The authors declare the study received no funding.

Conflict of interest

The authors declare that there is no conflict of interest.

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How to cite

1.
Büyük Yaytokgil Ş, Külhaş Çelik İ, Şengül Emeksiz Z, et al. Differences between multi-triggered and single-triggered food anaphylaxis in children: a real life study. Turk J Pediatr 2025; 67: 317-326. https://doi.org/10.24953/turkjpediatr.2025.5788